Coding

Part:BBa_K1085050:Design

Designed by: Mirjam Groenewold   Group: iGEM13_Groningen   (2013-10-04)


des knock out


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1234
    Illegal EcoRI site found at 3056
    Illegal PstI site found at 725
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1234
    Illegal EcoRI site found at 3056
    Illegal PstI site found at 725
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1234
    Illegal EcoRI site found at 3056
    Illegal BglII site found at 904
    Illegal BamHI site found at 2550
    Illegal XhoI site found at 3164
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1234
    Illegal EcoRI site found at 3056
    Illegal PstI site found at 725
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1234
    Illegal EcoRI site found at 3056
    Illegal PstI site found at 725
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

The part is derived from the genomic DNA of B. subtilis 168.

References